SPR Theory and Practice

J.A. Marquart • Boek • loose-leaf

• Samenvatting
  The aim of this book is to introduce biomolecular interaction analysis in combination with surface plasmon resonance. Both theory and practice will be discussed. The main emphasis will be on avoiding pitfalls and designing proper experiments. Although written with the BIACORE in mind, the general principles and guidelines will be applicable to comparable SPR instruments.
  Surface Plasmon Resonance (SPR) is a powerful technique to measure biomolecular interactions in real-time in a label free environment. While one of the interactants is immobilized to the sensor surface, the other are free in solution and passed over the surface. Association and dissociation is measured in arbitrary units and displayed in a graph called the sensorgram.
  Biomolecular Interaction Analysis is not limited to proteins. Interactions between DNA - DNA, DNA - protein, lipid - protein and hybrid systems of biomolecules and non-biological surfaces can be investigated.
  Biomolecular Interaction Analysis can be used in a number of ways. First, to identify the binding of two or more interactants to each other; second, to determine the affinity of the interactions; and third to measure the actual association and dissociation rates. In addition, the binding of two interactants can be exploited to quantify the concentration of one of the interactants.
• Productinformatie
  Binding : Loose-leaf
  Distributievorm : Boek (print, druk)
  Formaat : 145mm x 210mm
  Aantal pagina's : 287
  Uitgeverij : Niet bekend
  ISBN : Niet bekend
  Datum publicatie : 10-2013
  
• Inhoudsopgave
  1. Introduction to Biomolecular Interaction Analysis 1
  2. Kinetics 21
  3. Association 25
  4. Steady state 30
  5. Dissociation 36
  6. Regeneration 40
  7. Mass transfer 45
  8. Getting feeling for the curves 51
  9. Sensorchips 75
  10. Sample preparation 95
  11. Immobilization 100
  12. Experimanetal setup 127
  13. Data fitting 151
  14. Kinetic models 169
  15. Checklists 210
  16. Calculations 218
  17. References 230
  18. Resources 239
  19. Index 243
• Reviews (10 uit 3 reviews)
  Wil je meer weten over hoe reviews worden verzameld? Lees onze uitleg hier.

  
  

  17-04-2024
  CEO
  This is the best book about SPR I ever got. All of the rules, principles and practices are described in very good way, that is easy to understand. Adequate mathematics is given that is not overwhelming for the reader, but provides good, solid understanding. The book is helpful not only for the SPR, but for all other biophysical methods of molecular interaction nature, such as BLI, MST, LSPR, ICT, e.t.c. I would recommend every biochemist to have it.
  
  

     Illustractions
     Mathematics
     A pdf version would help also
  
  Geplaatst door Alexey Tomilov uit Davis, California, Bay Area , leeftijd 50-59
  Waardeert het boek met een 10 uit 10

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  25-04-2022
  Really happy with this book
  It is a very good guide to the Surface Plasmon Resonance understanding, with a very good approach to theory, enough and well synthetized, as well as a useful practical guide, very well designed and explained. I find it a very useful guide to the design of my SPR experiments. The book comes in handy wire-o binding.
  
  

     Content
     Language
     I haven´t find any yet
  
  Geplaatst door Maria A Corral uit Spain , leeftijd 40-49
  Waardeert het boek met een 10 uit 10

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  26-02-2019
  Really great
  I did my PhD on the maths of SPR, and its applications to medicine and this was my favourite book on SPR.
  
  

     thorough
     understandable
  
  Geplaatst door H.a.j.Moyse , leeftijd 30-39
  Waardeert het boek met een 10 uit 10